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. 2022 Jan 15;11(2):221. doi: 10.3390/plants11020221

Table 1.

List of abundantly altered protein profiles between well-watered, drought-stressed, and recovered Pandanus amaryllifolius.

Accession Protein Biological Process Function Cluster a
F1SWA0 Zerumbone synthase Protein synthesis Oxidoreductase 1
P49043 Vacuolar-processing enzyme Cysteine-type endopeptidase Hydrolase 1
P48711 Ribulose bisphosphate carboxylase large chain Photorespiration Magnesium ion binding 1
Q9FLN4 50S ribosomal protein L27, chloroplastic Ribonucleoprotein mRNA binding 1
A0A357 30S ribosomal protein S18, chloroplastic Ribonucleoprotein rRNA binding 1
A1E9N5 30S ribosomal protein S7, chloroplastic Ribonucleoprotein rRNA binding 1
O23760 Caffeic acid 3-O-methyltransferase Lignin biosynthesis Methyltransferase 1
B2LMP1 30S ribosomal protein S15, chloroplastic Ribonucleoprotein Structural constituent of ribosome 1
A2WXD9 Photosystem II 22 kDa protein 1, chloroplastic Photosynthesis Non-photochemical quenching 1
Q9XF91 Photosystem II 22 kDa protein, chloroplastic Photosynthesis Non-photochemical quenching 1
Q32RY4 30S ribosomal protein S4, chloroplastic Ribonucleoprotein rRNA binding 1
O24461 Ras-related protein Rab7 Protein transport GTPase activity 1
O22925 Vacuolar-sorting receptor 2 Protein transport Calcium ion binding 2
Q940M2 Alanine-glyoxylate aminotransferase 2 homolog 1, mitochondrial Photorespiration Aminotransferase 2
Q9LUI2 Protein NETWORKED 1A Cytoskeleton Actin binding protein 2
P43644 DnaJ protein homolog ANJ1 Stress response Chaperone 2
P11143 Heat shock 70 kDa protein Stress response Chaperone 2
A4QLY6 Photosystem I iron-sulfur center Photosynthesis (ET) Oxidoreductase 2
Q05737 GTP-binding protein YPTM2 Protein transport GTPase activity 2
Q04960 DnaJ protein homolog Stress response Chaperone 2
Q9XIM0 CCG-binding protein 1 Cellular response to hypoxia Mediator complex binding 3
P81370 Thaumatin-like protein Plant defence Pathogenesis 3
Q6DBP4 Pectin acetylesterase 8 Cell wall biogenesis/degradation Hydrolase 3
Q9FLC0 Peroxidase 52 Hydrogen peroxide Oxidoreductase 3
Q96520 Peroxidase 12 Hydrogen peroxide Oxidoreductase 3
P48980 Beta-galactosidase Carbohydrate metabolism Glycosidase 3
Q01289 Protochlorophyllide reductase, chloroplastic Chlorophyll biosynthesis Oxidoreductase 3
P26792 Beta-fructofuranosidase, insoluble isoenzyme 1 Carbohydrate metabolism Glycosidase 3
Q0DM51 DEAD-box ATP-dependent RNA helicase 3, chloroplastic Ribosome biogenesis Hydrolase 3
F6H7K5 Thiamine thiazole synthase 2, chloroplastic Thiamine biosynthesis Transferase 3
Q9LN49 3-ketoacyl-CoA synthase 4 Acyltransferase Fatty acid biosynthesis 3
Q75LR2 Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic Amino acid biosynthesis Transferase 3
O82627 Granule-bound starch synthase 1, chloroplastic/amyloplastic Starch biosynthesis Glycosyltransferase 3
Q8W0A1 Beta-galactosidase 2 Carbohydrate metabolism Glycosidase 3
O23787 Thiamine thiazole synthase, chloroplastic Thiamine biosynthesis Transferase 3
Q9ZQ94 UDP-glycosyltransferase 73C5 Brassinosteroid metabolism Glycosyltransferase 3
O80731 Pectin acetylesterase 3 Cell wall biogenesis/degradation Hydrolase 3
Q9C992 3-ketoacyl-CoA synthase 7 Acyltransferase Fatty acid biosynthesis 3
O22436 Magnesium-chelatase subunit ChlI, chloroplastic Chlorophyll biosynthesis Ligase 3
Q84P54 Gamma aminobutyrate transaminase 1, mitochondrial Biotin biosynthesis Aminotransferase 3
A5JTQ2 Beta-xylosidase/alpha-L-arabinofuranosidase 1 (Fragment) Carbohydrate metabolism Glycosidase 3
Q42850 Protochlorophyllide reductase B, chloroplastic Chlorophyll biosynthesis Oxidoreductase 3
Q9SD46 Peroxidase 36 Hydrogen peroxide Oxidoreductase 3
O04931 Alpha-glucosidase Carbohydrate metabolism Glycosidase 3
Q08937 29 kDa ribonucleoprotein B, chloroplastic mRNA processing Ribonucleoprotein 3
Q5ZE07 Multicopper oxidase LPR1 homolog 2 Phosphate homeostasis Oxidoreductase 3
A4S6Y4 Lon protease homolog, mitochondrial Oxidative stress DNA binding 3
Q40147 Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic Chlorophyll biosynthesis Isomerase 3
Q9LIK0 Plastidial pyruvate kinase 1, chloroplastic Glycolysis Kinase 3
Q6STH5 Fe-S cluster assembly factor HCF101, chloroplastic iron-sulphur cluster assembly 4Fe-4S cluster binding 3
Q0E3C8 Chaperone protein ClpB3, mitochondrial Stress response Chaperone 3
Q94LW3 Homeobox protein knotted-1-like 3 Mucilage biosynthesis DNA binding 3
Q42600 Cytochrome P450 84A1 Phenylpropanoid biosynthesis Monooxygenase 3
Q56UD0 Beta-fructofuranosidase, insoluble isoenzyme 6 Carbohydrate metabolism Glycosidase 3
Q8L7S6 Beta-hexosaminidase 3 Carbohydrate metabolism Glycosidase 3
Q39613 Peptidyl-prolyl cis-trans isomerase Protein folding Chaperone 3
Q9SJ20 Ribonucleoside-diphosphate reductase large subunit DNA replication Oxidoreductase 3
Q75GT3 Chaperone protein ClpB2, chloroplastic Stress response Chaperone 3
Q9ZUU4 RNA-binding protein CP29B, chloroplastic mRNA processing Ribonucleoprotein 3
Q9M591 Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic Chlorophyll biosynthesis Oxidoreductase 3
Q9CA67 Geranylgeranyl diphosphate reductase, chloroplastic Chlorophyll biosynthesis Oxidoreductase 3
P50246 Adenosylhomocysteinase One-carbon metabolism Hydrolase 3
Q6ZIV7 Hypersensitive-induced response protein 1 Potassium ion channel regulation Histidine kinase binding 3
Q9SI75 Elongation factor G, chloroplastic Protein biosynthesis Elongation factor 3
P24846 4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic Amino acid biosynthesis Allosteric enzyme 3
Q41932 Oxygen-evolving enhancer protein 3-2, chloroplastic Photosynthesis (ET) Calcium ion binding 4
P25795 Aldehyde dehydrogenase family 7 member A1 Stress response Oxidoreductase 4
Q9AXH0 Catalase Hydrogen peroxide Oxidoreductase 4
O65660 PLAT domain-containing protein 1 Stress response Catalase 4
A2YH64 Catalase isozyme B Hydrogen peroxide Oxidoreductase 4
Q0E4K1 Catalase isozyme A Hydrogen peroxide Oxidoreductase 4
O04932 Probable sucrose-phosphate synthase 1 Glycosyltransferase Sucrose biosynthesis 4
Q570C8 3-ketoacyl-CoA thiolase 5, peroxisomal Acyltransferase Fatty acid biosynthesis 4
Q9SG80 Alpha-L-arabinofuranosidase 1 L-arabinose metabolic Hydrolase 4

a Clusters 1–4 show the differential accumulation of differentially changed proteins (log ratio expression) between treatments (Figure S3B). Cluster 1 represents the decreasing protein abundance from the well-watered to drought-stressed and recovery samples. Cluster 2 shows the increasing protein abundance pattern from the well-watered to drought-stressed but decreasing from drought-stressed to recovery samples. Cluster 3 represents the decreasing protein abundance when comparing well-watered to drought-stressed but increasing from the drought-stressed to recovery samples. Cluster 4 shows the increasing abundance of proteins from well-watered to recovery samples.