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. 2022 Jan 10;70(2):577–583. doi: 10.1021/acs.jafc.1c07520

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© 2022 The Authors. Published by American Chemical Society

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iNOS and NO production in HepG2 cells treated with H2 or H3. Densitometric analyses of iNOS protein levels (A); representative Western Blots of iNOS in H2 (B) and H3 (C) assays; NO production (D). The data points represent the averages ± SD of three independent experiments in triplicate. All data sets were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test. Different letters indicate statistically significant differences. ****, p < 0.0001. C, unstimulated control group; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide-stimulated cells; NO, nitric oxide.