Table 1. Anti-/Pro-inflammatory Cytokines’ Ratioa.
C | LPS | H2 [100μM] | |
---|---|---|---|
IL10/IFN-γ | 1.05 ± 0.14a | 0.75 ± 0.15b | 1.10 ± 0.19a |
IL10/TNF | 0.99 ± 0.07a | 0.88 ± 0.05a | 1.14 ± 0.11b |
IL10/IL-6 | 1.01 ± 0.10a | 0.90 ± 0.03a | 0.96 ± 0.07a |
C | LPS | H3 [25μM] | |
---|---|---|---|
IL10/IFN-γ | 1.00 ± 0.03a | 0.93 ± 0.05a | 1.34 ± 0.06b |
IL10/TNF | 1.00 ± 0.02a | 0.92 ± 0.02a | 1.44 ± 0.12b |
IL10/IL-6 | 1.00 ± 0.02a | 0.90 ± 0.01a | 1.32 ± 0.13b |
Ratios between anti-inflammatory (IL-10) and pro-inflammatory (IFN-γ, TNF, and IL-6) cytokines quantified in HepG2 cells stimulated or not with LPS and treated with H2 (100 μM) or H3 (25 μM). Data are presented as mean ± SD and were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test. Different letters indicate statistically significant differences (p ≤ 0.05). C, unstimulated control group; IFN-γ, interferon-γ; IL, interleukin; LPS, lipopolysaccharide-stimulated cells; TNF, tumor necrosis factor.