Figure 1.

CRISPR/Cas9-mediated B7-H3 KO reduces the proliferation, migration and invasion of A549 cells. (A) Representative western blot (upper) and reverse transcription-quantitative PCR (lower) analysis for B7-H3 expression in B7-H3 KO and mock A549 cells. (B) The microscopic images of B7-H3 mock (left) and KO (right) A549 cells (magnification, ×200). (C) The proliferation of B7-H3 KO and mock A549 cells was determined by WST-8/Cell Counting Kit-8 at 0, 24, 48 and 72 h after cell culturing. Cell proliferation at 0 h was normalized to 1.0. ***P<0.001, ****P<0.0001. (D) Migration and (E) invasion of B7-H3 KO and mock A549 cells were assessed by Transwell system as indicated. Data are representative of three independent experiments. ns, no significance; KO, knockout.