Figure 3.

SIRT1 is involved in B7-H3-mediated epithelial-mesenchymal transition activation in A549 cells. (A) Western blot analysis for SIRT1 expression in B7-H3 KO and mock cells. (B) Western blotting (upper left) and the band intensity analysis (right), and reverse transcription-quantitative PCR (lower left) analysis for SIRT1 expression in siSIRT1 and siControl A549 cells as indicated. (C) Migration and (D) invasion of siSIRT1 and siControl A549 cells were assessed by Transwell system. Data are representative of three independent experiments. SIRT1, Sirtuin 1; KO, knockout; si, small interfering RNA.