Figure 5.

B7-H3 regulates SIRT1 expression via the PI3K/AKT signaling pathway. (A and B) Western blot and band intensity analysis for the t- and p-AKT, STAT3 and ERK1/2 in B7-H3 KO and mock A549 cells. (C-E) Western blot and band intensity analysis for t-AKT, p-AKT and SIRT1 expression in A549 cells treated with LY294002 (50 µΜ). GAPDH was used as the loading control, and relative phosphorylation level (p-/t-) was determined. Data are representative of three independent experiments. SIRT1, Sirtuin 1; t-, total; p-, phosphorylated; KO, knockout; ns, no significance; NC, negative control.