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. 2021 Dec 25;33(1):194–205. doi: 10.1021/acs.bioconjchem.1c00537

Figure 2.

Figure 2

In vitro characterization of N48, N49, N50, and N51. (A) IC50 values of ligands as determined in competitive binding assays using LS174T-PSMA cells. IC50 values were determined using a nonradiolabeled ligand (N48, N49, N50, and N51) in competition with 111In-labeled PSMA-617. Lipophilicity of ligands expressed in log D values. Internalization ratio as determined in LS174T-PSMA cells. (B) Membrane binding and internalization kinetics of N48, N49, N50, and N51 in LS174T-PSMA-positive and -negative cells. Nonspecific binding was determined by blocking with an excess of 2-PMPA (50 μg). PSMA-617 was added as a positive control.