Fig. (2).

NZD suppresses LPS-stimulated inflammatory response in RAW264.7 Cells. RAW264.7 cells were pretreated with NZD for 1 h and then stimulated with LPS (1 μg/mL) for 18 h. (A) The level of nitrite was determined by Griess reagent. (B, C) RAW264.7 cells were pretreated with NZD for 1 h and then stimulated with LPS (1 μg/mL) for 8 h. The level of NO was measured by a flow cytometer with a fluorescence probe DAF-FM. (D, E, F) The expressions of iNOS and COX-2 were detected by Western blotting. * p < 0.05, ** p <0.01, ***p < 0.001 vs. LPS group.