Figure 5.

An overview of the experimental design for assessing the automated sample preparation workflow for in vitro metabolomics. Extraction blanks and intrastudy QC samples were prepared on day 1 of the experiment, whilst biological control samples were prepared on days 2 and 3. Conditions tested included the location of a 96-well microplate (containing frozen cells for extraction) on the instrument’s deck (indicated as “a” or “b”), day and order of metabolite extraction as well as the location of samples within a microplate (edge vs. centre).