Table I.

Primers used in this study.

Gene	Sequence (5′→3′)	Genbank number	Size, bp	Tm, °C
GAPDH	F: GGTGGAGCCAAAAGGGTCA	XM_017321385.1	138	67.3
	R: GGGGGCTAAGCAGTTGGT			64.0
TRAP	F: AACTTGCGACCATTGTTA	XM_011242384.2	159	56.5
	R: GGGGACCTTTCGTTGATGT			63.7
Cathepsin K	F: GCAGAGGTGTGTACTATGA	BC046320.1	73	50.3
	R: GCAGGCGTTGTTCTTATT			57.8
RANKL	F: CCTGAGGCCCAGCCATTT	NM_011613.3	107	63.9
	R: CTTGGCCCAGCCTCGAT			66.5
TNF-α	F: CTGTAGCCCACGTCGTAGC	NM_013693.3	97	56.4
	R: TTGAGATCCATGCCGTTG			53.9

F, forward; R, reverse; Tm, temperature; TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of NF-κB ligand; TNF-α, tumor necrosis factor-α.