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. 2022 Jan 11;14(1):125. doi: 10.3390/v14010125

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Production of recombinant S1 (rS1) of porcine epidemic diarrhea virus (PEDV). (A) Polymerase chain reaction (PCR) amplificon of PEDV S1 gene (S1). Lane M, 1 kilobase (kb) DNA ladder. Numbers on the left are DNA sizes in base pairs (bp). (B) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separated pattern of the purified rS1 protein after staining with Coomassie Brilliant Blue G-250 (CBB) dye. (C) Western blot pattern of the rS1 as detected by anti-His antibody. Lanes M and numbers on the left of (B) and (C) are protein molecular mass marker and protein molecular masses in kilo-Daltons (kDa), respectively.