Figure 2.

MiniBio 500 bioreactor cultivation. LRS6 was cultivated in the Applikon Minibio 500 bioreactors in YPG prepared with yeast extract lot 175915. (A) The temperature ($\times$), pH ($o$) and dissolved oxygen (+) were monitored and controlled online to the set points of 30 °C, 6 and 30%, respectively. The biomass ($\diamond$) was monitored online using a BE2100 OD scanner (BugLab, Concord, CA, USA). The galactose consumption ($\square$) was monitored offline via sampling twice daily. (B) Total taxane (o) and diterpenoid 1 ($\diamond$) accumulation was measured via the GCMS analysis of dodecane extracts of offline samples. Oil immersion brightfield microscope images taken at 32 h (C) and at the end of the cultivation (D) using a 100× lens. (E) Biofilm formation observed on the bioreactor probes.