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(a) Typical nanoDSF thermogram showing the fluorescence intensity ratio at 330 nm and 350 nm; (b) Light scattering thermogram by measuring the attenuation of the backreflected light intensity passing through the sample as a function of temperature. The sample was IgG at a concentration of 10 mg/mL in the 50 mM sodium phosphate buffer at pH 7.4. Tonset—onset temperature of thermal unfolding; Tm—inflection temperature of thermal unfolding; Tagg—onset temperature of protein aggregation.
