Table 1.

Thermal stability of IgGs in three buffers with different pHs as measured by nanoDSF.

Buffers	pH	Thermal Stability Parameters (°C)
		T onset	T m	T agg
Sodium phosphate	5.0	57.9 ± 0.5	65.0 ± 0.0	74.0 ± 0.9
	5.2	58.7 ± 0.8	66.1 ± 0.0	73.6 ± 0.6
	5.4	59.9 ± 0.4	67.2 ± 0.1	71.8 ± 0.2
	5.6	60.7 ± 0.9	68.1 ± 0.0	70.1 ± 0.2
	5.8	61.2 ± 0.3	68.7 ± 0.0	69.4 ± 0.4
	6.0	61.4 ± 0.1	69.3 ± 0.1	68.5 ± 0.0
	6.2	61.6 ± 0.3	69.6 ± 0.0	68.5 ± 0.0
	6.4	61.8 ± 0.4	69.8 ± 0.0	68.2 ± 0.6
	6.6	61.4 ± 0.0	69.9 ± 0.1	69.1 ± 0.1
	6.8	60.1 ± 0.3	69.8 ± 0.1	69.3 ± 0.2
	7.0	60.8 ± 0.1	69.8 ± 0.1	69.1 ± 0.2
	7.3	60.4 ± 0.5	69.5 ± 0.1	69.5 ± 0.2
	7.5	60.2 ± 0.2	69.4 ± 0.1	70.1 ± 0.1
	7.7	59.7 ± 0.9	69.2 ± 0.0	69.2 ± 0.6
	8.0	58.9 ± 0.2	69.0 ± 0.0	70.5 ± 0.5
Sodium acetate	4.0	42.3 ± 0.1	51.4 ± 0.5	No aggregation
	4.2	45.0 ± 0.0	54.4 ± 0.1	No aggregation
	4.4	50.1 ± 0.5	58.0 ± 0.1	No aggregation
	4.6	53.2 ± 0.1	60.6 ± 0.1	No aggregation
	4.8	55.2 ± 0.2	62.8 ± 0.1	78.6 ± 0.4
	5.0	57.0 ± 0.3	64.6 ± 0.1	75.6 ± 1.6
	5.2	58.6 ± 0.1	66.1 ± 0.0	74.0 ± 0.2
	5.4	59.9 ± 0.1	67.2 ± 0.0	72.4 ± 0.1
	5.6	60.3 ± 0.5	68.2 ± 0.1	71.8 ± 0.2
	5.8	60.9 ± 0.3	69.0 ± 0.1	69.5 ± 0.2
	6.0	61.4 ± 0.3	69.7 ± 0.4	69.0 ± 0.3
Sodium citrate	4.0	40.1 ± 0.2	49.7 ± 0.2	67.6 ± 0.2
	4.2	44.3 ± 0.1	52.5 ± 0.2	65.5 ± 1.8
	4.4	47.6 ± 0.3	55.1 ± 0.1	65.3 ± 1.4
	4.6	50.5 ± 0.5	58.0 ± 0.1	66.2 ± 0.9
	4.8	53.0 ± 0.5	60.3 ± 0.1	66.0 ± 0.5
	5.0	55.4 ± 0.1	62.5 ± 0.0	67.6 ± 0.4
	5.2	57.7 ± 0.0	64.5 ± 0.1	67.8 ± 0.5
	5.4	58.4 ± 0.4	66.0 ± 0.1	67.8 ± 0.2
	5.6	59.7 ± 0.3	67.0 ± 0.0	68.1 ± 0.3
	5.8	60.2 ± 0.5	67.6 ± 0.0	68.9 ± 0.2
	6.0	60.6 ± 0.2	68.3 ± 0.0	69.0 ± 0.1

Values are the mean and standard deviation from triplicate measurements.