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. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Anal Chem. 2021 Feb 10;93(7):3643–3651. doi: 10.1021/acs.analchem.1c00276

Figure 2.

Figure 2.

Photostability comparison of secondary goat anti-rabbit IgG antibodies labeled with NIR dyes. Cells were incubated with primary anti-Tubulin and fluorescent secondary antibody (2’Ab-s775z, 2’Ab-CW800, or 2’Ab-DyLight800) followed by continuous irradiation with NIR light within a microscope equipped with an ICG filter (ex: 769/41, em: 832/37). Plots show normalized mean pixel intensity (MPI) for time-lapse cell micrographs.