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Multiplex imaging of HT-1080 cells. HT-1080 cells were fixed, co-incubated with primary rabbit anti-Tubulin and primary mouse anti-Actin overnight at 4 °C. Cells were co-incubated the following day with secondary goat anti-rabbit labeled with s775z (2’Ab-s775z) and secondary donkey anti-mouse labeled with FITC (2’Ab-FITC) for 2 hr at room temperature. Cells were co-stained with Hoechst 33342. Scale bar = 30 μm.
