Figure 5.

Comparison of secondary goat anti-rabbit IgG antibodies labeled with NIR dyes for targeting primary anti-Tubulin in western blots. HT-1080 cell lysate was run on a polyacrylamide SDS gel, and the separated proteins were transferred to a polyvinylidene fluoride membrane. The membrane was incubated with primary anti-Tubulin overnight at 4 °C and incubated with a fluorescent secondary antibody (2’Ab-s775z, 2’Ab-CW800 or 2’Ab- DyLight800), then a NIR fluorescence image of each α/β-Tubulin band was acquired.