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Inhibition assay for TMPRSS2, 3CLpro, and PLpro. (a) TMPRSS2 enzyme activity was measured using the following equation: A(I) = A(0) × {1 − [I/(I + IC50)]}. (b) 3CLpro enzyme activity was calculated with the Michaelis–Menten equation. (c) PLpro enzyme activity was calculated with the Michaelis–Menten equation.
