Figure 4.

RABV production was enhanced in MRC-5^IFNAR1− cells. (A) Genomic DNA from three MRC-5^IFNAR1−: gRNAs cell lines was extracted. The target sequence was amplified by PCR with IFNAR1 gRNA MRC-5^IFNAR1−-1,2,3 primers in Table 4 and then was sequenced. The deficiency of IFNAR1 in three MRC-5^IFNAR1− cell lines were confirmed by sequencing trace analysis. The gRNA target area is marked in gray. Mutated bases are marked in red, and—means deletion. (B) Three MRC-5^IFNAR1− cell lines, MRC-5^NC, and MRC-5 cells were cultured in 6-well plates to 80% confluence. Total RNA from cells was extracted, and the expression of IFNAR1 mRNA was detected by qRT-PCR. (C) Three MRC-5^IFNAR1− cell lines and MRC-5 cells were cultured in 6-well plates to 80% confluence. The cells were infected with the PM RABV strain at an MOI of 0.05. The total RNA from cells was extracted at 24 hpi, and the expression of IFN-β and OAS1 mRNA was detected by qRT-PCR. (D) Viral RNA in the culture supernatant were detected by qRT-PCR at 96 and 168 hpi. ⁺: Viral infection; ^NC: non-targeting control; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns p ≥ 0.05 compared with MRC-5⁺ at the indicated gene or time.