Figure 4.

Flagellar expression is decreased with higher levels of CbrR. TEM images of WT cells (A) possess two polar flagella, as expected, as do cbrR⁻ cells (B). However, overexpression of CbrR leads to the absence of flagella in cbrR⁺ cells (C). This was confirmed via Western blot probing for FlaA in cell lysate from each strain, which showed greatly decreased expression of FlaA in cbrR⁺ cells (D). Western blots using antibodies to FliF, which is expressed equally in these strains, confirmed equivalent loading of the samples.