Figure 5.

CbrR affects the extent of C. jejuni autoagglutination. AAG assays of WT, cbrR⁻, and cbrR⁺ over 24 h. The AAG assay measures the tendency of cells suspended in PBS to aggregate via the flagella over time by measuring the OD₆₀₀ in the top 1 mL of the 2 mL volume suspension of cells. The cbrR⁻ mutant shows a faster tendency to autoagglutinate when compared to WT, while the complemented cbrR⁺ shows delayed autoagglutination. All three groups were statistically different from 3 h onward (**** p < 0.0001). A one-way ANOVA with Tukey’s multiple comparisons test was used to determine statistical significance (GraphPad Prism 9, San Diego, CA, USA). This experiment was performed in triplicate and conducted three times.