Figure 10.

Mutation in the autoinhibitory site of CbrR leads to loss of c-di-GMP binding. Point mutants of cbrR to inactivate the I-site and active site were generated and their ability to bind GTP and c-di-GMP were established in DRaCALA assays. Results show that the CbrR active site mutant binds both GTP and c-di-GMP; however, the I-site mutant lost the capacity to bind c-di-GMP. The diguanylate cyclase Pseudomonas fluorescens WspR is shown as a positive control able to bind both small molecules while Pseudomonas aeruginosa Alg44 is a negative control for GTP binding but a positive control for c-di-GMP binding.