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. 2021 Dec 31;10(1):86. doi: 10.3390/microorganisms10010086

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LC-MS/MS shows low level amounts of c-di-GMP produced by C. jejuni CbrR. Chromatograms generated by LC-MS/MS show peak intensity of c-di-GMP for samples of C. jejuni cells: (A) WT Plate, (B) cbrR⁻ Plate, (C) cbrR⁺ Plate, (D) WT Biphasic, (E) cbrR⁻ Biphasic, (F) cbrR⁺ Biphasic, (G) WT Broth, (H) cbrR⁻ Broth, (I) cbrR⁺ Broth; and E. coli cells: (J) pCMW75 4 Hr, (K) pCMW98 4 Hr, (L) pCAF107 4 Hr, (M) pCAF109 4 Hr, (N) pCAF110 4 Hr, (O) pCMW75 O/N, (P) pCMW98 O/N, (Q) pCAF107 O/N, (R) pCAF109 O/N, (S) pCAF110 O/N. WT C. jejuni and recombinant E. coli (WT CbrR or CbrR_E334A) samples showed low level c-di-GMP production.