Table 1.

Detection of c-di-GMP in nucleotide extraction samples.

Sample	c-di-GMP (nM) in Cellular Extracts
WT Plate	0.4
cbrR− Plate	0
cbrR+ Plate	0
WT Biphasic	0
cbrR− Biphasic	0
cbrR+ Biphasic	0
WT Broth	0
cbrR− Broth	0
cbrR+ Broth	0
QrgB (pCMW75) 4 h expression	30.6
Inactive QrgB (pCMW98) 4 h expression	0
WT CbrR (pCAF107) 4 h expression	3.4
Active site mutant CbrR (pCAF109) 4 h expression	27.6
I-site mutant CbrR (pCAF110) 4 h expression	0
QrgB (pCMW75) O/N expression	816.0
Inactive QrgB (pCMW98) O/N expression	27.4
WT CbrR (pCAF107) O/N expression	10.9
Active site mutant CbrR (pCAF109) O/N expression	6.8
I-site mutant CbrR (pCAF110) O/N expression	4.0
c-di-GMP Standard (1.953 nM)	3.3
c-di-GMP Standard (3.906 nM)	4.2
c-di-GMP Standard (7.813 nM)	7.1
c-di-GMP Standard (15.625 nM)	15.6
c-di-GMP Standard (31.250 nM)	28.5
c-di-GMP Standard (62.500 nM)	63.8
c-di-GMP Standard (125.000 nM)	125.4
Blank	0