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. 2021 Dec 24;9(1):6. doi: 10.3390/vetsci9010006

Figure 5.

Figure 5

MyD88 is involved in RANKL-induced M cell differentiation. Murine intestinal organoids were established (A). Images of WT intestinal organoids were developed under a ZEISS Vert A1 microscope. Scale bar = 200 μm. Both WT and MyD88−/− organoids were stimulated with human recombinant RANKL for one day (PBS was used as control) and then were collected for RNA isolation. The mRNA levels of representative M-cell related markers, GP2, Spi-B, RANK, Tnfaip2, and CCL9, in both WT and MyD88−/− organoids, were detected by RT-qPCR (B). The fold change of these markers was compared after RANKL-treatment between WT and MyD88−/− group (C). The data were calculated using the 2−∆∆Ct method. In all graphs, data are shown as ‘mean ± SEM’. Results were designated with: * p < 0.05; ** p < 0.01; *** p < 0.001.