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. 2021 Dec 28;12(1):42. doi: 10.3390/life12010042

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Metabolism reprogramming characteristic of cancer cells cultured in anchorage independent condition. (A) GSEA for the enrichment of oxidative phosphorylation-related genes in HeLa cultured in suspension for 3 and 6 h compared to the control (adherent cells, 0 h); (B) GSEA for the enrichment of glucose metabolism-related genes of HeLa in suspension following ECM-detachment after 3 and 6 h compared to the control (attached cell, 0 h); (C,D) heat maps show the expression patterns for the genes contributing to core enrichment of oxidative phosphorylation and glucose metabolism; the indicated pathway in HeLa cultured in suspension after 3 h of cell detachment and adherent cells at 0 h as control.