Figure 1.

Expansion of CD4⁺CD8⁺ DP population with clinical plasma leakage in dengue disease. (A) Gating strategy to identify and sort CD4⁺CD8⁺ DP T cells from human PBMCs isolated from patients who were diagnosed at the acute phase of DENV infection (4–5 days since fever onset). (B) Representative flowcytometry plots demonstrate increased percentage of CD4⁺CD8⁺ DP T cells in dengue with plasma leakage (D+L) cohort compared to those in the dengue without plasma leakage (D-L) cohort. (C) Bar graph shows the frequencies of CD4⁺CD8⁺ DP T cells in D+L cohort (red, n = 55) and D-L cohort (grey, n = 33). Error bars show median with interquartile range. Statistical analysis was performed by two-tailed Mann–Whitney test, **** p < 0.0001.