Figure 2.

Transcriptomic analysis of CD4⁺CD8⁺ DP T cells compared with CD4⁺ and CD8⁺ SP T cells in acute DENV infection. (A) UMAP plot of bulk RNA-seq datasets from acute DENV infection samples colored by cell group (after QC: n = 64 for CD4⁺CD8⁺ DP T cell group, n = 88 for CD4⁺ SP T cell group, and n = 87 for CD8⁺ SP T cell group), where each dot represented one RNA-seq data from one individual. (B) Volcano plot shows log₂ fold change versus −log₁₀ p-value for the comparison between CD4⁺CD8⁺ DP T cells and CD8⁺ SP T cells. The subset of genes with log₂ fold change greater than 2 or less than −2 and adjusted p-value less than 0.05 are considered significant and indicated by dotted lines. (C) Heatmap shows the expression values after variance stabilizing transformation of the significant DE genes found between CD4⁺CD8⁺ DP T cells and CD8⁺ SP T cells. (D) Functional pathway enrichment analysis using g:Profiler for differentially expressed genes between CD4⁺CD8⁺ DP and CD8⁺ SP T cells; p_adj value means adjusted p value.