Figure 3.

Comparison of transcriptomic signatures of CD4⁺CD8⁺ DP T cells from dengue with plasma leakage (D+L) cohort and dengue without plasma leakage (D-L) cohort. (A) UMAP plot of bulk RNA-seq datasets from acute DENV infection samples colored by disease groups (after QC: n = 41 for D+L group, n = 23 for D-L group), where each dot represented one RNA-seq data from one individual. (B) Volcano plot shows log₂ fold change versus −log₁₀ p-value for the comparison between CD4⁺CD8⁺ DP T cells from D+L and D-L groups. The subset of genes with log₂ fold change greater than 2 or less than −2 and adjusted p-value less than 0.05 are considered significant and indicated by dotted lines. (C) Heatmap shows the expression values after variance stabilizing transformation of the significant DE genes found between CD4⁺CD8⁺ DP T cells from D+L and D-L groups.