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Viability, confluence and quantification of ECs adherent to HFM. Viable ECs on AH (A) and FN (B) coated HFM were visualized using calcein staining (green); Scale: 200 µm, VE-cadherin (green) and Hoechst (blue) indicated intercellular connection between ECs on AH (C) and FN (D) coated HFM; Scale: 100 µm. (E) Quantification of adherent ECs on AH and FN coated HFM, ECs on non-coated (NC) HFM were not detectable (N.D.).
