Skip to main content
. 2022 Jan 10;15(1):83. doi: 10.3390/ph15010083

Figure 2.

Figure 2

Optimization of the different extraction and compound loading methods for the exosomes. (A) The sizes (diameter, nm) of the exosomes obtained by the three different methods were measured and compared by NTA. (B) UHPLC-MS was adopted to detect the herbal Hed concentration of the exosomes loaded by the three selected compound cargo loading methods (EDL, FH and RT). (C) Pictures were captured to show the presence of the precipitated Exo and Exo-Hed vacuoles extracted by the SDUC method. (D) Pictures were captured to show the presence of the precipitated Exo and Exo-Hed vacuoles extracted by the UC method. (E) The Exo concentration and the Hed concentration of the seven fractions obtained by SDUC were examined by NTA and UHPLC-MS, respectively. (F) The Exo concentration and Hed concentration obtained by SDUC and UC were detected by NTA and UHPLC-MS, respectively. All of the experiments were performed as three independent experiments. One-way ANOVA was used to compare the data, * p < 0.05.