Figure 8.

Exo-Nef effectively improved the motor behavioral deficiency of APP/PS1 mice and reduced the Aβ deposition in the mouse brain. (a) A rotarod test was used to observe the behavior of APP/PS1 double transgenic mice as described in the methodology section. (b) After the mice were sacrificed, the brain tissues were collected for further Western blot analysis on the level of the Aβ fibril normalized with β-actin. All of the experiments were performed as three independent experiments. (c) A dot blot analysis on the protein level of total Aβ in the mouse brain of different treatment groups was performed. (d) Pharmacokinetic study of neferine with or without the encapsulation by an exosome. (e) Schematic diagram showing the use of an exosome as a carrier for the selected neuroprotective herbal compound for crossing the blood–brain barrier in an AD mouse model. One-way ANOVA was used to compare the data: * p < 0.05, *** p < 0.001.