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. 2021 Dec 30;14(1):60. doi: 10.3390/v14010060

Figure 7.

Figure 7

GPS491 alters the function of only a subset of SR proteins. (a) Map depicting the Bcl-x pre-mRNA and the Bcl-x minigene 2.13 as well as the position of the primers used for RT-PCR. (b) Map of the endogenous Bcl-x gene and spliced products Bcl-xS and Bcl-xL. Positions of primers for RT-PCR are indicated. (c) HEK 293 cells were transfected with Bcl-x reporter along with plasmids expressing the indicated SR proteins, followed by treatment with DMSO or 10 μM GPS491. After 48 h, total RNA was isolated and RT-PCR was performed. The relative abundance of the xL and xS products was determined following fractionation on PAGE gels. (d) Assays were repeated with overexpression of SRSF10 in the presence of DMSO and 10 or 20 µM GPS491, evaluating the effect on alternative splicing of the endogenous Bcl-x gene (Bcl-x ENDO). Representative gel images are provided in Supplementary Figure S3.