FIG. 1.

Quantitation of RT activity by the STF-PERT assay. (A) RT activity of MV vaccine lot 12 (□) and lot 29 (▴) and MVVE (●) determined in triplicate by STF-PERT analysis of serial dilutions of each sample. (B) Standard linear plot of AMV RT activity, derived by STF-PERT analysis of serial dilutions, tested in triplicate, containing the indicated amount of RT except for dilution with 1E+10 pU, which was tested in duplicate. Means and standard deviations of RT activity versus the threshold cycle (Ct) are shown.