FIG. 3.

Analysis of integration of avian retrovirus sequences in MVVE-incubated human PBMCs. (A) PCR analysis done to detect the presence of EAV, ALV, and CRE sequences in DNA prepared from human PBMCs incubated with MVVE (with and without DNase treatment) at 24 h postexposure. PCR primers are indicated in the legend to Fig. 2B. (B) DNA PCR of PBMCs incubated with MVVE in the presence of AMLV as a positive control for retrovirus infection using AMLV F1-AMLV R1. (C) RT-PCR analysis of MVVE-incubated PBMCs done to demonstrate infection of PBMCs by MV using primers indicated in the legend to Fig. 2B. Lanes 1, uninoculated PBMC DNA control in panels A and B and PCR control in panel C; lanes 2, MVVE material inoculated without DNase treatment; lanes 3, MVVE material inoculated with DNase treatment. Ten microliters of amplified products (panel B and β-actin panel) or reamplified products (panels A and C) was analyzed on a 1.4% agarose gel by ethidium bromide staining of DNA. The fragments expected are indicated (in base pairs). The 838-bp fragment amplified with human β-actin primers is shown.