TABLE 1.

Oligonucleotides used for DNA PCR and RT-PCR

PCR primer	Sequence (5′ → 3′)	Nucleotide position	Fragment size (bp)
EAV F1	GCTGTTCTTCATGTGCGAAG	1624–1643a	352
EAV R1	CAGTGGACGCGGTATCAACAGTAA	1975–1951a
EAV F2	GTTCCGGGATTCTTTACAACTG	1654–1675a	276
EAV R2	CAGACGAGGCTCCAGCGTGAAGTC	1929–1906a
ALV 21F	TGTGTGGATTGACCAGTGG	2789–2807b	301
ALV 21R	CGCAAGAGGAATAGAAAAGAAG	3068–3089b
ALV P1	CCTGTCTTTGTGATCCGG	2904–2921b	159
RSV R1	CTTGAGGTCTAAGACCATCAG	3062–3042c
CRE F1	GACTGGATGAGTACAAGTCTTC	2997–3018d	393
CRE R1	CAAGTCCAGGTAAATGACATC	3389–3409d
CRE F2	GTCAGGCGAGGTCCCAGATGAC	3109–3130d	268
CRE R2	CTCATCCACCAACGCCGTCAC	3376–3356d
MNP1	GGTTCGGATGGTTCGAGAACA	428–448e	477
MNP2	GGTTCATCAAGGACTCAAGTG	884–904e
MNP3	TGAAGTGCAAGACCCTGAGGG	465–485e	400
MNP4	TTCATGCAGTCCAAGAGCAGG	844–864e

^aFrom reference 10. 

^bBased upon endogenous ALV sequences in CEF cells (37). 

^cGenBank accession no. J02342, J02021, and J02343 (32). 

^dGenBank accession no. U88211 (13). 

^eFrom reference 12.