Skip to main content
. 2022 Jan 11;14(1):126. doi: 10.3390/v14010126

Figure 4.

Figure 4

Interferon regulatory factor 7 (IRF7) was a target gene of miR-541-3p. HEK 293T cells were transfected with miR-541-3p mimics (A), miR-541-3p inhibitors (B), NC-mimics or NC-inhibitors. Then, 48 h later, the expression of predicted target mRNAs of miR-541-3p was measured by quantitative real time polymerase chain reaction (qRT-PCR). HEK 293T cells were transfected with miR-541-3p mimics at a final concentration of 50 nM or 100 nM (C), miR-541-3p inhibitors at a final concentration of 50 nM or 100 nM (D) and NC-mimics or NC-inhibitors. Then, 48 h later, the expression levels of IRF7 were measured by qRT-PCR. (E) Schematic presentation of base pairing between the 3′ untranslated region (UTR) of IRF-7 and the miR-541-3p sequence; the underlined red bases are their paired bases and their corresponding mutant bases. HEK 293T cells were transfected with the pcDNA6.2-miR-541-3p or pcDNA6.2-Mut-miR-541-3p and psiCHECK-2-IRF7-3′-UTR or psiCHECK-2-Mut-IRF7-3′-UTR, and 24 h later, the cells were harvested for luciferase activity assay by using the dual-luciferase reporter assay system. All experiments were repeated at least three times with similar results. * p < 0.05; ** p < 0.01.