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. 2022 Jan 11;14(1):126. doi: 10.3390/v14010126

Figure 6.

Figure 6

Interferon regulatory factor 7 (IRF7) inhibited the replication of PRRSV-2. MARC-145 cells were transfected with siIRF7 or siNC, and 48 h later, the protein levels (A) and the mRNA expression levels (B) of IRF7 were assessed by Western blots and quantitative real time polymerase chain reaction (qRT-PCR), respectively. (C) MARC-145 cells were transfected with siIRF7, and 24 h later, the cells were infected with PRRSV-2, and then the cells were harvested and the RNA levels of Open reading frame 7 (ORF7) of PRRSV-2 were determined by qRT-PCR. (D) MARC-145 cells were transfected with pCMV-IRF7-Flag, and 24 h later, the protein expression levels of IRF7 were assessed by Western blots. (E) MARC-145 cells were transfected with pCMV-IRF7-Flag, and 24 h later, the cells were infected with PRRSV-2, and then the cells were harvested and the RNA levels of PRRSV-2 ORF7 were determined by qRT-PCR. All experiments were repeated at least three times with similar results. ** p < 0.01.