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. 2022 Jan 9;11(2):167. doi: 10.3390/plants11020167

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression and purification of recombinant protein TrufOBP4 analyzed by SDS-PAGE. (A) SDS-PAGE analysis of the expression of recombinant TrufOBP4. M: Protein molecular weight marker. 1: Protein sample without IPTG induction; 2: Protein sample with 0.5 mM IPTG induction. 3: The supernatant of protein sample with 0.5 mM IPTG induction after ultrasonic crushing. 4: The precipitation of protein sample with 0.5 mM IPTG induction after ultrasonic crushing. (B) SDS-PAGE analysis of the purification of recombinant TrufOBP4. M: Protein molecular weight marker. 1: Protein fluid after flowing through the column. 2–6: Protein samples were obtained with imidazole elution at different concentrations (0, 40, 100, 200 and 1000 mmol/L imidazole). The target protein was marked by an arrow.