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. 2022 Jan 16;14(1):160. doi: 10.3390/v14010160

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Interaction of HHV-6B gB with nectin-2. (A) The 293T cells were co-transfected with pCAGGS-HST-gB-HA or pCAGGS-HST-gQ1-HA and pCAGGS-nec2δ-FLAG or pCAGGS-FLAG. Cell lysates were subjected to immunoblotting (IB) and immunoprecipitation (IP) with the indicated antibodies. HHV-6B gB consists of three polypeptides of 102 kDa, and its proteolytic cleavage products of 59 and 50 kDa [52]. C-terminal HA-tagged recombinant gB proteins were expressed and used. (B) Recombinant nectin-2-Fc was immunoprecipitated with soluble HST-gB-HA or HA. (C) HHV-6B-infected MT-4 cells were cultured for 6 d and lysates were subjected to co-IP with anti-HHV-6 gB antibody (6A5). Mock-infected MT-4 cells were used as a negative control. (D) 293T cells were co-transfected with pCAGGS-gB (HST or Z29)-HA or pCAGGS-HA and pCAGGS-nec2δ-FLAG or pCAGGS-FLAG. Cell lysates were subjected to IB with the indicated antibodies (left panel). Cell lysates were subjected to IP with anti-FLAG or anti-HA antibodies (right panel). WCE, whole-cell extract.