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. 2001 Feb;39(2):685–690. doi: 10.1128/JCM.39.2.685-690.2001

FIG. 1.

FIG. 1

Schematic representation of the small ribosomal subunit 18S gene. The hatched boxes correspond to the polymorphic region from V3 to V7. The black boxes correspond to the highly polymorphic region of V4 (nucleotides 679 to 690, 697 to 717, and 725 to 731 in Saccharomyces cerevisiae numbering). Primer locations: i) TR1 and TR2 primers, described by Bock et al. as dermatophyte specific (2, 3); FaR and FaL, used to sequence the V4 domain of T. soudanense, M. langeronii, Scopulariopsis brevicaulis, S. dimidiatum, and S. hyalinum; and DH1L-DH1R and DH2L-DH1R, used respectively to amplify dermatophytes and Scytalidium spp.