Figure 2.

Animal body weight data and assay conditioning. A) Body weight gains across experimental groups. B) Coomassie stains of purified biosensor and CaM preparations. C) Spectral change of biosensor in response to CaM titration. D) Biosensor fractional saturation as a function of CaM in the absence (open squares) or presence (closed triangles) of 530 μL of flow-through from Step 1, Fig. 1A. Total assay volume, biosensor, Ca²⁺ and CaM concentration were identical in the two conditions. *, p < 0.05; **, p < 0.01; ****, p < 0.0001. Data are means ± SD from n = 8 – 10 animals for A; n = 3 for each condition in D.