Figure 3.

Effect of experimental menopause and estrogen replacement on unoccupied CaM-binding proteins in LV tissue. A) Relative BSCaM₄₅ responses to CaM titration in the absence (CTL, closed circles) or presence of 530 μL eluants (Step 3, Fig. 1A) from equal protein contents (Step 1, Fig 1A) of LV lysates from sham or OVX animal treated with 17β-estradiol (E₂) for 2 weeks. Total assay volume, biosensor, Ca²⁺ and CaM concentration were identical across the groups. B) Relative unoccupied CaM-binding proteins (uoCBPs) calculated from biosensor responses in A at corresponding 25% (open columns), 50% (filled columns) and 75% (hatched columns) of control biosensor responses (dashed lines in A). *, p < 0.05; *, p <0.01; ns, non-significant. Data are means ± SD from n = 5 repetitions for each condition.