Figure 1.

Schematic of the thermal gradient experimental setup. A temperature gradient was created on an aluminum plate (1) using a cold and a warm water bath on either side, connected with copper tubing (2). The mosquitoes were released into acrylic covers (3) through a hole in the top with a fitted acrylic plug (4). Each cover had moist wipe towels (5) and holes drilled on each side to minimize humidity build up (6). The acrylic enclosures are open on their bottom side to allow direct contact of mosquitoes with the aluminum plate.