Figure 3.

Thermopreference in Culex mosquitoes. (A). Mosquito distribution (bottom) on the aluminum plate set at 25 °C (i.e., constant temperature, control) and corresponding density plot (top). (B). Mosquito distribution (bottom) on the low temperature gradient (bottom) and corresponding density plot (top). (C). Mosquito distribution (bottom) on the high temperature gradient (bottom) and corresponding density plot (top). The density plots summarize the mosquitoes’ distribution along the gradient. Each dot represents the final resting position (i.e., T_p) of a single female mosquito. The boxes represent the upper and lower quartiles, and the black bars indicate the mean of each group. n.s. denotes mosquito distributions in the constant gradient experiments that were not significantly different from a uniform, continuous distribution (Kolmogorov–Smirnov tests, α = 0.05). Letters denote statistical differences between groups (Tukey post hoc tests for multiple comparisons, adjusted α = 0.05). Four replicates (n = 10; N = 40) have been used for the constant gradient. Ten replicates (n = 10, N = 100) per species have been used for the low and high gradients.