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Gradient HPLC method for quantification of cysteamine and cystamine. The mobile phase A was an aqueous sodium heptane sulfonate solution (pH 2.5) and the mobile phase B was acetonitrile.
| Time (min) | Mobile Phase A (%) | Mobile Phase B (%) |
|---|---|---|
| 0 | 80 | 20 |
| 1.0 | 80 | 20 |
| 16.0 | 35 | 65 |
| 16.1 | 10 | 90 |
| 19.0 | 10 | 90 |
| 19.5 | 80 | 20 |
| 24.0 | 80 | 20 |
