TABLE 2.
Results of melting analysis among 186 patients examined with the LightCycler
| Results obtained by melting analysis
|
Results obtained by RFLP analysisa
|
||||||
|---|---|---|---|---|---|---|---|
| Wild type | A2144G | A2143G | No. (%) of patientsb | BsaI | MboI | Uncut band | No. of patients |
| + | − | − | 119 (78.8) | − | − | + | 119 |
| − | + | − | 17 (11.2) | + | − | − | 17 |
| − | − | + | 1 (0.66) | − | + | − | 1 |
| + | + | − | 10 (6.6) | + | − | + | 10 |
| + | − | + | 1 (0.66) | − | + | + | 1 |
| − | + | + | 1 (0.66) | + | + | − | 1 |
| + | + | + | 2 (1.33) | + | + | + | 2 |
| Subtotal | 151 | 151 | |||||
| − | − | − | 35 | − | − | − | 35 |
| Total | 186 | 186 | |||||
a
BsaI, PCR products can be cut by BsaI (presence of the mutant with the A2144G mutation); MboI, PCR products can be cut by MboI (presence of the mutant with the A2143G mutation); uncut band, presence of uncut band even after treatment with both restriction enzymes (presence of the wild type).
b
Values in parentheses indicate the percentage of patients in whom H. pylori was detected with the LightCycler.