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. 2022 Jan 11;10(1):102. doi: 10.3390/vaccines10010102

Figure 3.

Figure 3

Anti-Sda1 serum prevented NETs degradation by Sda1. (A) Mouse anti-Sda1 serum neutralized GAS M1 culture supernatant and wild-type (Wt)-Sda1-mediated degradation of plasmid DNA. GAS M1 culture supernatant and Wt-Sda1 in the presence of an immunized serum in the plasmid degradation assay. Anti-Sda1 serum or naïve serum was pre-mixed with GAS M1 culture supernatant or Wt-Sda1 in the plasmid degradation assay. (B) Anti-Sda1 serum IgG neutralized Wt-Sda1-mediated degradation of neutrophil NETs. Mouse neutrophils were isolated and incubated for 4 h without stimulation (lane 1) or with PMA stimulation (lanes 2, 3, 4, 5). Released neutrophil NETs after PMA stimulation were visualized by DAPI (blue) staining. Aliquots of Wt-Sda1 were pre-incubated with the indicated rabbit serum IgG before their addition to PMA-stimulated neutrophils. Representative images from two to three experiments are shown. Scale bar, 10 mm. Abbreviations: sup., GAS culture supernatants.