Fig 6.

Liner epitopes with high binding intensity and declining reactive frequencies over time, recognized by SARS-CoV-2–infected individuals. A and B, Longitudinal analysis and distribution of identified peptides exhibiting high binding signal intensity (above mean + SD of signal intensities of all tested samples) but decreasing positive rate over time. The recognition frequency (A) and signal intensity (B) of epitopes were plotted with 3 sampling time points after symptom onset. Each dot in (B) stands for an individual patient serum sample collected at indicated time points after symptom onset; dotted horizontal lines indicate cutoff values of positivity for each peptide. Statistical significance analysis was performed based on Limma of R v3.6.3 software. ∗P < .05 and ∗∗P < .01. C, Location and sequence comparisons of 2 adjacent epitopes, peptide 510 (dominant and persistent) and peptide 511 (high signal intensity and decreasing positivity over time), on SARS-CoV-2 S protein structure (PDB ID: 6ZGI). The overlapping regions between 2 epitopes are highlighted in green; unique sequences are labeled in red and blue.