Figure 3.

Transport of radiolabeled carboxylates in C. jadinii DSM2361 strain (A–C), S. cerevisiae jen1Δ ato1Δ (A,B), and IMX1000 (C) cells expressing the C. jadinii CjATO1-5, CjJEN1-6, CjSLC16, CjSLC5, CjTDT, CjSLC13-1, and CjSLC13-2 proteins, as well as an empty vector (pɸ), as negative control. (A) Uptake of 1 mM of ¹⁴C-acetic acid (pH 6.0), ¹⁴C-lactic acid (pH 5.0), and ¹⁴C-succinic (pH 5.0) acid. For acetic and lactic acid uptake, a value of 100% (C+) corresponds to the uptake in S. cerevisiae cells transformed with the plasmid p416GPD-expressing genes encoding the native transporters ATO1 and JEN1, respectively. For succinic acid, a value of 100% (C+) corresponds to isogenic cells expressing the Candida albicans JEN2 transporter cloned in the p416GPD plasmid. (B) Uptake of 1 mM ¹⁴C-acetic acid (pH 6.0) and 1 mM ¹⁴C- lactic acid (pH 5.0). A value of 100% corresponds to the uptake of acetic and lactic acid displayed by cells expressing the JEN1 transporter. (C) Uptake of 1 mM of ¹⁴C-acetic acid (pH 6.0). A value 100% corresponds to the uptake of acetic acid by C. jadinii DSM 2361 cells. Cells were grown on YNB Glucose, washed, and incubated on YNB containing the respective carbon source (see materials and methods) used in the uptake assay for 6 h (acetate 0.5%, pH 6.0) or 5 h (lactate 0.5%, pH 5.0; succinate 1%, pH 5.0).